Part:BBa_K274111:Design
CrtY under Pbad promoter (I0500)
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). BBa_K2741x0 series (BBa_K274100, BBa_K274110 and BBa_K274120) contain enzyme cassette CrtEBI that convert farnesyl pyrophosphate to lycopene (red). BBa_K274111 (I0500+CrtY) then converts lycopene (red) to beta-carotene (orange).
Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment production than strain TOP10.
Source
The (rbs+CrtY) component comes from BBa_K118013. The coding sequence for CrtY comes from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087).
The Pbad promoter comes from BBa_I0500.